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EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format,

貨號(hào):21329,名稱:EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format,品牌:PIERCE,規(guī)格:8 X 2 MG
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EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format介紹

 

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

 

 

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EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format現(xiàn)貨供應(yīng)

 

PIERCE

21329

EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format,

8 X 2 MG

PIERCE

21331

EZ-Link (tm) Sulfo-NHS-SS-Biotin, 100 mg

100 MG

PIERCE

21335

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg

100 MG

 

EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format品牌介紹

 

美國(guó)Pierce公司(www.pierce.com)是蛋白質(zhì)化學(xué)、免疫學(xué)和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

 

主要產(chǎn)品包括:

1)化學(xué)發(fā)光體和比色底物

 

2)分析試劑

 

3)熒光標(biāo)記探針

 

4)酶聯(lián)免疫分析試劑盒等

 

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用于快速純化組氨酸標(biāo)簽蛋白。

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25個(gè)柱

 

EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format

僅用于科研

 

Thermo Scientific EZ-Link NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.

The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. The hydrophilic polyethylene glycol (PEG) spacer arm of this biotinylation reagent imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution (read more about PEGylation). The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

Highlights:

  • Label almost any protein with biotin to facilitate immobilization, purification or detection
  • Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
  • Reagent is water soluble and membrane impermeable – suitable for cell surface labeling
  • PEGylation increases solubility and helps prevent aggregation of labeled molecules
  • Long spacer arm reduces steric hindrance when binding to avidin molecules
  • No-Weigh Format saves time, preserves reagent activity and prevents cross-contamination between uses
  • Reactive group: N-hydroxysuccinimide ester (NHS ester)
  • Reactive toward: primary amines, as in side chain of lysine (K) and N-terminus of polypeptides



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