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EZ-Link （R） Biotin-PEG4-Hydrazide介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

EZ-Link （R） Biotin-PEG4-Hydrazide現(xiàn)貨供應

品牌介紹

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主要產(chǎn)品包括：

1）化學發(fā)光體和比色底物

2）分析試劑

3）熒光標記探針

4）酶聯(lián)免疫分析試劑盒等

僅用于科研

Thermo Scientific EZ-Link Hydrazide-PEG4-Biotin （also called Biotin-PEG4-Hydrazide） is an aldehyde-reactive biotinylation reagent that contains a 4-unit polyethylene glycol （PEG） spacer arm for increased hydrophilicity.

Biotin-PEG4-Hydrazide enables biotinylation of glycoproteins and other molecules containing aldehydes or oxidizable sugar groups. The hydrazide group reacts spontaneously with aldehyde or ketone groups to form a stable hydrazone bond. Aldehyde groups can be created by gentle periodate oxidation of cis-diols in sialic acid and other sugars in carbohydrates. The hydrophilic, 4-unit polyethylene glycol （PEG） spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG segment also adds length and flexibility to the spacer arm, minimizing steric hindrance involved with binding to avidin molecules.

Highlights:

• Carbohydrate-reactive – forms stable hydrazone bond with periodate-oxidized sialic acid and other sugar groups in glycoproteins
• Good choice for labeling polyclonal antibodies in the carbohydrate-rich Fc region, thereby avoiding steric hindrance of the antigen-binding sites
• Use to biotinylate cell surface glycoproteins
• PEGylation increases solubility and helps prevent aggregation of labeled molecules
• PEG spacer arm reduces steric hindrance when binding to avidin molecules
• Reactive group: hydrazide

• Reactive toward: aldehyde and ketone groups （oxidized cis-diols in glycoproteins）