PIERCE，N-Succinimidyl 3-（2-Pyridyldithio）Propionat
- 型號： 21857
- 更新時間： 2024-05-31
- 上一個：21667PIERCE，DIMETHYL PIMELIMIDATE. 2HCl（DMP）
- 下一個：22122PIERCE，Sulfo-SMCC, 1 g.
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N-Succinimidyl 3-（2-Pyridyldithio）Propionate （SPDP）介紹
Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。
N-Succinimidyl 3-（2-Pyridyldithio）Propionate （SPDP）現(xiàn)貨供應(yīng)
PIERCE | 21857 | N-Succinimidyl 3-（2-Pyridyldithio）Propionate （SPDP） | 50 MG |
PIERCE | 22122 | Sulfo-SMCC, 1 g. | 1 G |
PIERCE | 22305 | MPBH: 4-（4-N-Maleimidophenyl）Butyric Acid Hydrazide Hydrochl | 50 MG. |
N-Succinimidyl 3-（2-Pyridyldithio）Propionate （SPDP）品牌介紹
美國Pierce公司（www.pierce.com）是蛋白質(zhì)化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html
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1）化學發(fā)光體和比色底物
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N-Succinimidyl 3-（2-Pyridyldithio）Propionate （SPDP）
僅用于科研
Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable （reducible） disulfide bonds with cysteine sulfhydryls.
Thermo Scientific Pierce LC-SPDP is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable （reducible） disulfide bonds with cysteine sulfhydryls.
We offer three analogs of SPDP: the standard version （SPDP）, a derivative with a longer spacer arm （LC-SPDP） and Sulfo-LC-SPDP, the sulfonated analog of LC-SPDP is available for applications that require a water-soluble, membrane-impermeable crosslinker. SPDP （N -Succinimidyl 3-（2-pyridyldithio）-propionate） and LC-SPDP （Succinimidyl 6-（3-[2-pyridyldithio]-propionamido）hexanoate） are heterobifunctional, thiol-cleavable and membrane permeable crosslinkers. They each contain an amine-reactive N-hydroxysuccinimide （NHS） ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.
Highlights:
- Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
- Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
- Cleavable crosslinker allows separation of crosslinked products
- SPDP crosslinker is membrane permeable, so crosslinking can be carried out inside the cell
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