重組人成纖維細(xì)胞生長(zhǎng)因子-9(rHuFGF-9)
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- 更新時(shí)間: 2024-05-23
- 上一個(gè):重組人成纖維細(xì)胞生長(zhǎng)因子-22(rHuFGF-22)
- 下一個(gè):重組人堿性成纖維細(xì)胞生長(zhǎng)因子/(bfgf/rHuFGF-b)
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產(chǎn)品介紹
CYT-415 | 重組人成纖維細(xì)胞生長(zhǎng)因子-9(rHuFGF-9) | 10μg/50μg/1mg | Prospec |
Description : | |
Recombinant Human FGF-9 produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 23 kDa. Recombinant FGF-9 is purified by proprietary chromatographic techniques. The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a single, potential N-linked glycosylation site. The native protein is glycosylated and is efficiently secreted after synthesis, although FGF -9 lacks a typical secretion signal. Rat and mouse FGF-9 show a very high homology to human FGF-9. The transcripts for FGF-9 have been found in brain and in kidney tissue. | |
Physical Appearance: | |
Sterile Filtered white lyophilized powder. | |
Formulation: | |
The sterile protein powder is lyophilized with no additives. | |
Solubility: | |
It is recommended to reconstitute the lyophilized Recombinant FGF-9 sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
Stability: | |
Lyophilized Human FGF-9 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Human FGF-9 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | |
Purity: | |
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
Amino acid sequence: | |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Leu-Gly. | |
Dimers and aggregates: | |
Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
Biological Activity: | |
ProSpec’s Recombinant FGF-9 is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 5.5 x 106 Units/mg. | |
Endotoxin: | |
Less than 0.1 ng/µg (IEU/µg) of Recombinant Human Fibroblast Growth Factor-9. | |
Protein content: | |
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.8 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of FGF-9 as a Reference Standard. | |
Usage: | |
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
佰易聚生物商城提供美國(guó)光譜醫(yī)學(xué)透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優(yōu)質(zhì)的分子生物學(xué)試劑和試劑盒、培養(yǎng)基本和培養(yǎng)耗材、細(xì)胞因子和細(xì)胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現(xiàn)貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購(gòu)!
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