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重組人干細胞因子(sf-9來源)(rHuSCF-Sf9)

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CYT-421重組人干細胞因子(sf-9來源)(rHuSCF-Sf9) 2μg/10μg/1mg Prospec

 

 

Description :
 Recombinant Human SCF produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.
SCF is fused to a C-terminal His-tag (6x His) and purified by proprietary chromatographic techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

 
Solubility:
 It is recommended to reconstitute the lyophilized Recombinant Human SCF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
 Lyophilized SCF although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Human SCF should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
 
Purity:
 Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
 ProSpec’s Recombinant SCF-Sf9 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 5x105 IU/mg.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of SCF-Sf9.

 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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